国家卫生健康委员会天然药物生物合成重点实验室-九游官网下载

科研队伍

孔建强,男,博士,研究员,博士生导师,课题组长。主要从事天然药物的生物合成及合成生物学研究。发表文章80余篇,申请专利十余项(授权5项)。《药学学报》,《acta pharmaceutica sinica b》青年编委,《生物工程学报》编委。《acta pharmaceutica sinica b》, 《acs synth biol》,《microbial cell fact》,《bioresource technology》,《bmc plant biology》,《biochimie》,《journal of agricultural and food chemistry》,《phytochemistry》,《药学学报》和《生物工程学报》等三十余种中外期刊的审稿专家。

1,天然产物生物合成
挖掘参与活性天然产物生物合成相关的途径酶基因,并对其进行功能表征;通过计算机模拟以及定向进化等手段对关键途径酶进行突变,获得活性显著提升,具有预设催化特性的生物催化剂,用于天然产物的酶法合成。
2,合成生物学技术制备天然产物
在不同底盘细胞中重构天然产物生物合成代谢途径及调控网络,获得能制备天然产物的细胞工厂,用于天然产物的生物合成。

1, xue-ning wang, li-li hong, and jian-qiang kong*, diacerein as a promising acyl donor in biosynthetic acetyl-coa and glycosyl esters mediated by a multifunctional maltose o‑acetyltransferase from escherichia coli, j. agric. food chem, 2021, 69, 6623−6635
2, li-li hong and jian-qiang kong*, altering the regioselectivity of cytochrome p450 bm3 variant m13 towards genistein through protein engineering and variation of reaction conditions, acs omega, 2020, 5, 49, 32059-32066
3, yan-li xu and jian-qiang kong*, ocugt1-catalyzing glycodiversification of steroids through glucosylation and transglucosylation actions. molecules 2020, 25, 475.
4, qing-wei chen, ting-gong, pei-cheng zhang, jian-qiang kong*, seven new 1-oxygenated cholestane glycosides from ornithogalum saundersiae, journal of asian natural products research, 2020, 22(3):201-206.
5, yu-jia sun, jiu-ming he and jian-qiang kong*, characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from ornithogalum caudatum jacq, bmc plant biology, 2019, 19:195.
6, qing-wei chen, xu zhang, ting gong, wan gao, shuai yuan, pei-cheng zhang*, jian-qiang kong*, structure and bioactivity of cholestane glycosides from the bulbs of ornithogalum saundersiae baker. phytochemistry, 2019, 164: 206-214.
7, ming liu, jian-qing kong*, the enzymatic biosynthesis of acylated steroidal glycosides and their cytotoxic activity. acta pharmaceutica sinica b, 2018, 8(6):981-994.
8, shuai yuan, sen yin, ming liu, jian-qiang kong*, isolation and characterization of a multifunctional flavonoid glycosyltransferase from ornithogalum caudatum with glycosidase activity. scientific reports, 2018, 8, 5886.
9, shuai yuan, sen yin, ming liu, jiu-ming he, jian-qiang kong*, ocugt1-catalyzed glycosylation of testosterone with alternative donor substrates, process biochemistry, 2018,73:82-85.
10, shuai yuan, yan yang, jian-qiang kong*, biosynthesis of 7,8-dihydroxyflavone glycosides via ocugt1-catalyzed glycosylation and transglycosylation, journal of asian natural products research, 2018, 20(7):662-674.
11, xin liu, jian-qiang kong*, steroids hydroxylation catalyzed by the monooxygenase mutant 139-3 from bacillus megaterium bm3, acta pharmaceutica sinica b, 2017, 7(4):510–516.

1.孔建强,尹森,一种来源于虎眼万年青的尿苷-5’-二磷酸木糖差向异构酶,其核苷酸序列及应用,zl201610147497.9,2020年3月31日授权
2.孔建强,尹森,一种来源于虎眼万年青的尿苷-5’-二磷酸半乳糖差向异构酶,其核苷酸序列及应用,zl201610147498.3,2020年3月31日授权
3.朱平,王伟,孔建强,戴均贵,程克棣,王丽娜,郑晓东,生产紫穗槐-4,11-二烯和青蒿酸的方法,2012,中国,zl2007101879228

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